SANGER

Sequencing (NGS and Sanger)

03-SANG-27

EQA for quality of Sanger sequencing (PCR products and primers provided)

Overview

This scheme is designed to assess the analytical process

(genotyping), reporting (and variant nomenclature) and quality of raw data. It is designed to be suitable for laboratories regardless of their routinely sequenced genes.

At a glance

Product Code:

03-SANG-27

Target/Genes:

Sanger DNA sequencing (gene independent)

If you are planning to participate in the DNA Sequencing – SANGER EQA scheme, you have the option to participate in this EQA scheme for Quality ONLY, which is designed to assess the quality of raw data submitted alongside relevant technical information.

Additionally, you have the option to participate in this EQA scheme for Quality AND Genotyping, which is designed to assess the analytical process (genotyping), reporting (diagnostic interpretation and variant nomenclature) and quality of raw data.

This is available for selection at the purchase stage when ‘Add to Basket' is selected. There is no additional cost for either option. For more information or to change your order please contact office@emqn.org

Technology:

Sanger sequencing

Sample Type:

PCR product (from g.DNA)

Submit Results In:

Not applicable

Accredited:

Yes

Key Dates

Register by

01/03/2027

Samples ship

Jun-27

Survey Period

June - August 2027

Final Results

December 2027 - January 2028

Sample Details

Five samples (including one control sample) plus two primers

(N13 Forward and N13 Reverse) are provided for sequencing.

Complete information for samples will be available in the

scheme instructions.

EQA Results

Participants will receive an individual report including educational advice and a scheme report which includes additional information from the cohort of participants including geographical spread, methodologies employed, common errors, learning points and scheme statistics allowing participants to benchmark their results.

Each laboratory will also receive a single certificate listing all EQAs participated in with performance result for each year.

Participation Information

Participants are expected to test samples using the provided N13 Primers according to their routine strategy, to upload base called (processed) sequencing data files for each sample and to complete the results submission form. Please note: Completion of the mandatory results submission form is compulsory in order to provide essential quality information.

This EQA is NOT suitable for labs using workflows where N13 primers cannot be utilised (e.g. M13 or custom primers). If your laboratory is interested in a scheme for M13 primers – please email office@emqn.org to register interest.

No restrictions on number of participants. Open to laboratories from ALL countries (note: some restrictions may apply due to sanctions, delivery network exceptions, restrictions on importation etc).

Send your enquiry by submitting the form

Closes in 112 days

From £519.00

Buying is completed on the EMQN portal and needs a free EMQN account, which takes a few minutes to set up.

Add to enquiry list
  • ISO 17043 accredited scheme

Questions about this scheme?
Contact our team

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