EQA for quality of Sanger sequencing (PCR products and primers provided)
This scheme is designed to assess the analytical process
(genotyping), reporting (and variant nomenclature) and quality of raw data. It is designed to be suitable for laboratories regardless of their routinely sequenced genes.
03-SANG-27
Sanger DNA sequencing (gene independent)
If you are planning to participate in the DNA Sequencing – SANGER EQA scheme, you have the option to participate in this EQA scheme for Quality ONLY, which is designed to assess the quality of raw data submitted alongside relevant technical information.
Additionally, you have the option to participate in this EQA scheme for Quality AND Genotyping, which is designed to assess the analytical process (genotyping), reporting (diagnostic interpretation and variant nomenclature) and quality of raw data.
This is available for selection at the purchase stage when ‘Add to Basket' is selected. There is no additional cost for either option. For more information or to change your order please contact office@emqn.org
Sanger sequencing
PCR product (from g.DNA)
Not applicable
Yes
01/03/2027
Jun-27
June - August 2027
December 2027 - January 2028
Five samples (including one control sample) plus two primers
(N13 Forward and N13 Reverse) are provided for sequencing.
Complete information for samples will be available in the
scheme instructions.
Participants will receive an individual report including educational advice and a scheme report which includes additional information from the cohort of participants including geographical spread, methodologies employed, common errors, learning points and scheme statistics allowing participants to benchmark their results.
Each laboratory will also receive a single certificate listing all EQAs participated in with performance result for each year.
Participants are expected to test samples using the provided N13 Primers according to their routine strategy, to upload base called (processed) sequencing data files for each sample and to complete the results submission form. Please note: Completion of the mandatory results submission form is compulsory in order to provide essential quality information.
This EQA is NOT suitable for labs using workflows where N13 primers cannot be utilised (e.g. M13 or custom primers). If your laboratory is interested in a scheme for M13 primers – please email office@emqn.org to register interest.
No restrictions on number of participants. Open to laboratories from ALL countries (note: some restrictions may apply due to sanctions, delivery network exceptions, restrictions on importation etc).
Closes in 112 days
From £519.00
Buying is completed on the EMQN portal and needs a free EMQN account, which takes a few minutes to set up.
Questions about this scheme?
Contact our team
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